ncode multi-species mirna microarray kit v2  (Thermo Fisher)

Journal: PLoS ONE

Article Title: Over-Expression of miR-106b Promotes Cell Migration and Metastasis in Hepatocellular Carcinoma by Activating Epithelial-Mesenchymal Transition Process

doi: 10.1371/journal.pone.0057882

Figure Lengend Snippet: Candidate miRNAs identified from miRNA microarray analysis.

Article Snippet: Tagged miRNAs were then purified and hybridized onto NCode Multi-Species miRNA Microarray V2.0 (Life Technologies, Carlsbad, CA), containing probes for Sanger mirBASE 9.0, overnight at 52°C.

Techniques: Microarray

Journal: BMC Genomics

Article Title: Cross-platform analysis of global microRNA expression technologies

doi: 10.1186/1471-2164-11-330

Figure Lengend Snippet: Within platform correlations for miRNA arrays.

Article Snippet: Invitrogen NCode Multi-Species miRNA Microarray Probe Set V2 , 34-44 nucleotide probes designed as dimers of sequences complementary to mature miRNAs, trimmed to reduce melt temperature variability between probes. , MirBase 9 , Two , 118 , 0.706 , 0.0544 , 0.586-0.798.

Techniques: Microarray, Oligo Synthesis, Binding Assay

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Gastric Cancer MicroRNAs Meta-signature

doi: 10.22088/IJMCM.BUMS.8.2.94

Figure Lengend Snippet: Characteristics of included studies

Article Snippet: Oleg T ( 8 ) , Germany , 476 , 6 (3 vs. 3) , 20726036 , Invitrogen NCode Multi-Species miRNA Microarray.

Techniques: Microarray

Journal: Oncology Letters

Article Title: MicroRNA-23a/27a/24-2 cluster promotes gastric cancer cell proliferation synergistically

doi: 10.3892/ol.2018.8924

Figure Lengend Snippet: miR expression pattern in gastric cancer. (A) Heat map showing expression levels of the indicated miRNA in relation to tissue type. The color represents the expression level of the microRNA, according to the color key shown below the heat map. Red represents high expression; blue represents low expression. (B) The structure of the pre-miRNA-transcript of miR-23a/27a/24-2. mi, micro; miR, microRNA.

Article Snippet: MicroRNA analyses were commissioned by the High-throughput Genome Analysis Core Facility of the VYM Genome Research Center (National Yang-Ming University, Taipei, Taiwan) on NCode TM Multi-Species miRNA microarrays (Invitrogen; Thermo Fisher Scientific, Inc.).

Techniques: Expressing

Journal: Oncology Letters

Article Title: MicroRNA-23a/27a/24-2 cluster promotes gastric cancer cell proliferation synergistically

doi: 10.3892/ol.2018.8924

Figure Lengend Snippet: SOCS6 is a target gene of miR-27a and miR-24. (A) The putative binding sites of miR-23a, miR-27a and miR-24 on SOCS6 were predicted using Targetscan. The interspecies sequence alignment revealed the mature miRNA sequences and the putative binding sites. Luciferase reporter constructs containing WT or mutant target sites of the 3′-untraslated region of SOCS6 mRNA for miR-23a, miR-27a and miR-24, respectively. (B) SC-M1 cells were co-transfected with dual luciferase reporter plasmid, containing a specific miRNA-binding sequence (wild-type or mutant), and 90 nM miR inhibitor for 24 h. Dual-luciferase activities were measured using a microplate reader. *P<0.05 and **P<0.01 for mutant vs. wild-type. (C) SC-M1 cells were treated with miR-23a, miR-27a and miR-24 inhibitors mixture for 48 h. The expression of SOCS6 was examined by western blotting. SOCS6, suppressor of cytokine-induced signaling 6; miR, microRNA; WT, wild-type.

Article Snippet: MicroRNA analyses were commissioned by the High-throughput Genome Analysis Core Facility of the VYM Genome Research Center (National Yang-Ming University, Taipei, Taiwan) on NCode TM Multi-Species miRNA microarrays (Invitrogen; Thermo Fisher Scientific, Inc.).

Techniques: Binding Assay, Sequencing, Luciferase, Construct, Mutagenesis, Transfection, Plasmid Preparation, Expressing, Western Blot

Journal: FEBS Open Bio

Article Title: Small RNA expression and deep sequencing analyses of the nucleolus reveal the presence of nucleolus-associated microRNAs

doi: 10.1016/j.fob.2014.04.010

Figure Lengend Snippet: MiRNA expression profiling of nucleolar RNA. (A) Small RNA isolation and analysis by 16% denaturing PAGE. Amount of RNA loaded is indicated at the top. (B, C) MiRNA expression analysis using NCode Multi-Species array. (B) HeLa ( N = 41 miRNA); (C) MCF7 ( N = 48 miRNA). Scatter plot of nucleolar and total RNA intensities are shown. The nucleolar intensities were adjusted according to snoRNA expression in the respective total cellular and nucleolar fraction. Scatter plots are thresholded at 8 (HeLa) and 6 (MCF7) to exlude low intensity miRNAs. (D) Top-ranking nucleolar miRNAs in HeLa and MCF7 cells. (E, F) Scatter plots of HeLa and MCF7 nucleolar (E) and cellular (F) miRNA intensities ( N = 36 miRNA). Pearson correlation coefficiencies ( r ) are indicated in each plot.

Article Snippet: The samples were hybridized in triplicate to NCode™ Multi-Species miRNA Microarray (Invitrogen) overnight according to the instructions by the manufacturer.

Techniques: Expressing, Isolation